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id7000 software  (Sony Biotechnology)


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    Structured Review

    Sony Biotechnology id7000 software
    Configuration of <t>ID7000</t> The SONY ID7000 is equipped with five lasers (355□ nm, 405 □nm, 488 □nm, 561 □nm, and 637□ nm) and 149 detectors, enabling detection of the full fluorochrome emission spectrum.
    Id7000 Software, supplied by Sony Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 801 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/analyzer+software/ID7000+Spectral+Cell+Analyzer/bio_rxiv__64898__2026__10__13__758716-91-9-11
    Average 99 stars, based on 801 article reviews
    id7000 software - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay"

    Article Title: A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay

    Journal: bioRxiv

    doi: 10.64898/2026.10.13.758716

    Configuration of ID7000 The SONY ID7000 is equipped with five lasers (355□ nm, 405 □nm, 488 □nm, 561 □nm, and 637□ nm) and 149 detectors, enabling detection of the full fluorochrome emission spectrum.
    Figure Legend Snippet: Configuration of ID7000 The SONY ID7000 is equipped with five lasers (355□ nm, 405 □nm, 488 □nm, 561 □nm, and 637□ nm) and 149 detectors, enabling detection of the full fluorochrome emission spectrum.

    Techniques Used:


    Figure Legend Snippet: Instrument configuration for the SONY 5 laser ID7000. Sony ID7000 flow cytometer laser detection channels and fluorescent antibodies. The antibodies and fluorophores used in this panel are indicated on the corresponding detectors.

    Techniques Used: Flow Cytometry

    Unmixing and Adjustments: N×N plots viewed with the ID7000™ Unmixing Viewer. Unmixing was performed on the live cell population. Unmixed data with B220-BUV496 depicted as an example on the x-axis and all other fluorochromes plotted on the y-axis. Examples of unmixing errors and corrected unmixing results are indicated by red boxes
    Figure Legend Snippet: Unmixing and Adjustments: N×N plots viewed with the ID7000™ Unmixing Viewer. Unmixing was performed on the live cell population. Unmixed data with B220-BUV496 depicted as an example on the x-axis and all other fluorochromes plotted on the y-axis. Examples of unmixing errors and corrected unmixing results are indicated by red boxes

    Techniques Used:

    Related Articles

    Software:

    Article Title: Discovery of 1-(3-phenoxybenzyl)-4-(1-phenyl-1H-1,2,3-triazol-4-yl)piperidine analogues as potent and selective agonists of the human CC chemokine receptor 8.
    Article Snippet: The human chemokine receptor 8 (CCR8) plays a role in various autoimmune disorders, such as multiple sclerosis and inflammatory bowel disease, spurring the interest in CC8 agonism as a potential therapeutic strategy.. Triazolyl substituted phenoxybenzylpiperidine analogues have been previously synthesized and were shown to act as CCR8 agonists although with moderate potency.. In this study, their structure-activity relationship was expanded by the synthesis of a series of 1,4-disubstituted 1,2,3-triazole analogues with structural modifications of the phenoxybenzylpiperidinyl and phenyl moieties.

    Article Title: Natural senolytic activity of Rhodiola rosea extract alleviates age-associated phenotypes via paraptosis
    Article Snippet: .. ID7000 software (version 1.1.0.11041) , Sony, Japan , N/A. .. Living Image software (version 4.5.5) , Perkin Elmer, USA , N/A.

    Article Title: A preinvasive regulatory T cell axis for lung cancer interception.
    Article Snippet: BD FACS Aria Fusion and BD Aria III for cell sorting (BD Bioscience). .. Sony Biotechnology ID7000 software for spectral flow cytometry using ID7000 Spectral Cell Analyser (Sony Biotechnology). .. Illumina NovaSeq 6000 for scRNA-seq using 10x Genomics kits.

    Article Title: A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay
    Article Snippet: .. All flow cytometry data was acquired or analyzed using ID7000 software (Sony Biotechnology). ..

    Article Title: ERα blockade in dendritic cells enhances antigen cross-presentation and induces antitumor CD8 + T cell immunity.
    Article Snippet: We used the CellTraceTM CFSE Cell Proliferation Kit (C34554; Thermo) for CFSE staining according to the manufacturer’s protocol. .. For analysis of mouse DC isotype, 1×106 DCs were blocked with TruStain FcXTM PLUS (anti-mouse CD16/32) antibodies at 4 °C for 10 min, then stained with PE or APC-anti-mouse CD11c antibody, FITCanti-mouse CD80 antibody, PE-Cy7-anti-mouse CD86 antibody, APC or PE anti-mouse H2Kb bound to SIINFEKL antibody and FITC anti-mouse I-A/I-E antibody for 30 min. For analysis of human DC isotype, 1×106 DCs were stained with BV421 anti-human HLA-DR antibody, PECF594 or BV605-anti-human CD86 antibody, BV510-anti-human CD40 antibody and PE-Cy7anti-human CD274 antibody for 30 min. Stained cells were analyzed by ID7000 (SONY) BD LSRFortessa or BD FACSymphony A1 flow cytometer and analyzed using FlowJo version 10 software. ..

    Article Title: Δ133p53α, a senescence-inhibitory p53 isoform, attenuates accelerated aging in progeria mice
    Article Snippet: T lymphocytes were isolated by negative selection using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies, #19851). .. Flow cytometry was performed according to standard procedures to detect CD3 expression using an anti-CD3-BUV395 antibody (BD Biosciences, #563565) and EGFP fluorescence in the FITC/EGFP channel, followed by data acquisition using a Sony ID7000 spectral cell analyzer and data analysis using FlowJo software. ..

    Article Title: CD163 and Tim-4 identify resident intestinal macrophages that are spatially regulated by TGF-β
    Article Snippet: For intracellular antibody staining, fixed cells were permeabilized with Permeabilization Buffer (Thermo Fisher Scientific) and stained with CD63 (NVG-2) and CD206 (C068C2) from BioLegend and pro-IL-1β (NJTEN3), TNF-α (MP6-XT22), and iNOS (CXNFT) from Thermo Fisher Scientific. .. Cell acquisition was performed on an LSRFortessa running FACSDIVA software (BD) or SONY ID7000 (SONY). ..

    Flow Cytometry:

    Article Title: A preinvasive regulatory T cell axis for lung cancer interception.
    Article Snippet: BD FACS Aria Fusion and BD Aria III for cell sorting (BD Bioscience). .. Sony Biotechnology ID7000 software for spectral flow cytometry using ID7000 Spectral Cell Analyser (Sony Biotechnology). .. Illumina NovaSeq 6000 for scRNA-seq using 10x Genomics kits.

    Article Title: A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay
    Article Snippet: .. All flow cytometry data was acquired or analyzed using ID7000 software (Sony Biotechnology). ..

    Article Title: ERα blockade in dendritic cells enhances antigen cross-presentation and induces antitumor CD8 + T cell immunity.
    Article Snippet: We used the CellTraceTM CFSE Cell Proliferation Kit (C34554; Thermo) for CFSE staining according to the manufacturer’s protocol. .. For analysis of mouse DC isotype, 1×106 DCs were blocked with TruStain FcXTM PLUS (anti-mouse CD16/32) antibodies at 4 °C for 10 min, then stained with PE or APC-anti-mouse CD11c antibody, FITCanti-mouse CD80 antibody, PE-Cy7-anti-mouse CD86 antibody, APC or PE anti-mouse H2Kb bound to SIINFEKL antibody and FITC anti-mouse I-A/I-E antibody for 30 min. For analysis of human DC isotype, 1×106 DCs were stained with BV421 anti-human HLA-DR antibody, PECF594 or BV605-anti-human CD86 antibody, BV510-anti-human CD40 antibody and PE-Cy7anti-human CD274 antibody for 30 min. Stained cells were analyzed by ID7000 (SONY) BD LSRFortessa or BD FACSymphony A1 flow cytometer and analyzed using FlowJo version 10 software. ..

    Article Title: Δ133p53α, a senescence-inhibitory p53 isoform, attenuates accelerated aging in progeria mice
    Article Snippet: T lymphocytes were isolated by negative selection using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies, #19851). .. Flow cytometry was performed according to standard procedures to detect CD3 expression using an anti-CD3-BUV395 antibody (BD Biosciences, #563565) and EGFP fluorescence in the FITC/EGFP channel, followed by data acquisition using a Sony ID7000 spectral cell analyzer and data analysis using FlowJo software. ..

    Staining:

    Article Title: ERα blockade in dendritic cells enhances antigen cross-presentation and induces antitumor CD8 + T cell immunity.
    Article Snippet: We used the CellTraceTM CFSE Cell Proliferation Kit (C34554; Thermo) for CFSE staining according to the manufacturer’s protocol. .. For analysis of mouse DC isotype, 1×106 DCs were blocked with TruStain FcXTM PLUS (anti-mouse CD16/32) antibodies at 4 °C for 10 min, then stained with PE or APC-anti-mouse CD11c antibody, FITCanti-mouse CD80 antibody, PE-Cy7-anti-mouse CD86 antibody, APC or PE anti-mouse H2Kb bound to SIINFEKL antibody and FITC anti-mouse I-A/I-E antibody for 30 min. For analysis of human DC isotype, 1×106 DCs were stained with BV421 anti-human HLA-DR antibody, PECF594 or BV605-anti-human CD86 antibody, BV510-anti-human CD40 antibody and PE-Cy7anti-human CD274 antibody for 30 min. Stained cells were analyzed by ID7000 (SONY) BD LSRFortessa or BD FACSymphony A1 flow cytometer and analyzed using FlowJo version 10 software. ..

    Cytometry:

    Article Title: Δ133p53α, a senescence-inhibitory p53 isoform, attenuates accelerated aging in progeria mice
    Article Snippet: T lymphocytes were isolated by negative selection using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies, #19851). .. Flow cytometry was performed according to standard procedures to detect CD3 expression using an anti-CD3-BUV395 antibody (BD Biosciences, #563565) and EGFP fluorescence in the FITC/EGFP channel, followed by data acquisition using a Sony ID7000 spectral cell analyzer and data analysis using FlowJo software. ..

    Expressing:

    Article Title: Δ133p53α, a senescence-inhibitory p53 isoform, attenuates accelerated aging in progeria mice
    Article Snippet: T lymphocytes were isolated by negative selection using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies, #19851). .. Flow cytometry was performed according to standard procedures to detect CD3 expression using an anti-CD3-BUV395 antibody (BD Biosciences, #563565) and EGFP fluorescence in the FITC/EGFP channel, followed by data acquisition using a Sony ID7000 spectral cell analyzer and data analysis using FlowJo software. ..

    Fluorescence:

    Article Title: Δ133p53α, a senescence-inhibitory p53 isoform, attenuates accelerated aging in progeria mice
    Article Snippet: T lymphocytes were isolated by negative selection using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies, #19851). .. Flow cytometry was performed according to standard procedures to detect CD3 expression using an anti-CD3-BUV395 antibody (BD Biosciences, #563565) and EGFP fluorescence in the FITC/EGFP channel, followed by data acquisition using a Sony ID7000 spectral cell analyzer and data analysis using FlowJo software. ..

    Analysis:

    Article Title: Δ133p53α, a senescence-inhibitory p53 isoform, attenuates accelerated aging in progeria mice
    Article Snippet: T lymphocytes were isolated by negative selection using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies, #19851). .. Flow cytometry was performed according to standard procedures to detect CD3 expression using an anti-CD3-BUV395 antibody (BD Biosciences, #563565) and EGFP fluorescence in the FITC/EGFP channel, followed by data acquisition using a Sony ID7000 spectral cell analyzer and data analysis using FlowJo software. ..

    other:

    Article Title: Setup and Analysis of Human Tonsil and Adenoid‐Based Immune Organoids for Studying T and B Cell‐Mediated Adaptive Immunity
    Article Snippet: Immune organoids in culture (from Basic Protocol ) Cell culture medium (see recipe) 1× PBS (Thermo Fisher Scientific, cat. no. 14190169) Zombie NIR fixable viability kit (BioLegend, cat. no. 423106) Flow cytometry buffer (see recipe) Human TruStain FcX (BioLegend, cat. no. 422302) Normal mouse serum (Invitrogen, cat. no. 10410; alternatively, Merck, cat. no. NS03L) Antibodies for flow cytometry (see Table ) BD Horizon Brilliant Stain Buffer Plus (BD, cat. no. 566385) eBiosciences FoxP3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, cat. no. 00‐5523‐00) Compensation beads (BioLegend, cat. no. 424602) Incubator (with 5% CO , 37°C) 96‐well cell culture plate, suspension, U‐bottom (Sarstedt, cat. no. 83.3925.500) 96‐well V‐bottom micro test plate (Sarstedt, cat. no. 82.1583) Centrifuge (Eppendorf, cat. no. 5811000015) 200‐μl multichannel piston pipette (Mettler Toledo, cat. no. 17013810) 200‐μl pipette tips (Mettler Toledo, cat. no. 30389240) 15‐ml centrifuge tube (Greiner Bio‐One, cat. no. 188271) 1‐ml piston pipette (Mettler Toledo, cat. no. 17014382) 1‐ml pipette tips (Mettler Toledo, cat. no. 30389223) Eppendorf tubes (Eppendorf, cat. no. 0030121023) Heat block ThermoMixer C (Eppendorf, cat. no. EP5382000015) Ice bucket and ice 20‐μl piston pipette (Mettler Toledo, cat. no. 17011788) 20‐μl pipette tips (Mettler Toledo, cat. no. 30389225) Sony ID7000 spectral analyzer (Sony Biotechnology) or similar Sony ID7000 analyzer software (Sony Biotechnology) or similar FlowJo v.10 (Waters)



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    Image Search Results


    Configuration of ID7000 The SONY ID7000 is equipped with five lasers (355□ nm, 405 □nm, 488 □nm, 561 □nm, and 637□ nm) and 149 detectors, enabling detection of the full fluorochrome emission spectrum.

    Journal: bioRxiv

    Article Title: A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay

    doi: 10.64898/2026.10.13.758716

    Figure Lengend Snippet: Configuration of ID7000 The SONY ID7000 is equipped with five lasers (355□ nm, 405 □nm, 488 □nm, 561 □nm, and 637□ nm) and 149 detectors, enabling detection of the full fluorochrome emission spectrum.

    Article Snippet: All flow cytometry data was acquired or analyzed using ID7000 software (Sony Biotechnology).

    Techniques:

    Journal: bioRxiv

    Article Title: A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay

    doi: 10.64898/2026.10.13.758716

    Figure Lengend Snippet: Instrument configuration for the SONY 5 laser ID7000. Sony ID7000 flow cytometer laser detection channels and fluorescent antibodies. The antibodies and fluorophores used in this panel are indicated on the corresponding detectors.

    Article Snippet: All flow cytometry data was acquired or analyzed using ID7000 software (Sony Biotechnology).

    Techniques: Flow Cytometry

    Unmixing and Adjustments: N×N plots viewed with the ID7000™ Unmixing Viewer. Unmixing was performed on the live cell population. Unmixed data with B220-BUV496 depicted as an example on the x-axis and all other fluorochromes plotted on the y-axis. Examples of unmixing errors and corrected unmixing results are indicated by red boxes

    Journal: bioRxiv

    Article Title: A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay

    doi: 10.64898/2026.10.13.758716

    Figure Lengend Snippet: Unmixing and Adjustments: N×N plots viewed with the ID7000™ Unmixing Viewer. Unmixing was performed on the live cell population. Unmixed data with B220-BUV496 depicted as an example on the x-axis and all other fluorochromes plotted on the y-axis. Examples of unmixing errors and corrected unmixing results are indicated by red boxes

    Article Snippet: All flow cytometry data was acquired or analyzed using ID7000 software (Sony Biotechnology).

    Techniques: